Viridi Vivid is added to the molten agarose before pouring the gel. After electrophoresis, the gel can be viewed immediately without any need to incubate in stain. The sensitivity is about 0.25 ng DNA / band and the effects on migration rates are minimal, even at higher DNA loads.
The DNA samples are first run out on an agarose gel. This approach avoids the possibility of any DNA-stain interactions on migration rates and is recommended for the most accurate estimation of DNA band size. Subsequent to electrophoresis, the gel is incubated for 30 minutes in a bath of Viridi Vivid. The post-staining method offers the most sensitive detection – less than 0.1 ng DNA / band.