|``We find that the dye is highly compatible with the Dark Reader and particularly recommend your instrument for investigators who manually excise bands from gels for proteomic investigations. Spots may be excised from gels with no hazard to personnel from UV injury and no photobleaching of the stain.``|
|Wayne F. Patton, Ph.D.
Group Leader, Bioanalytical Assay Development
Molecular Probes, Inc.
|SYPRO Ruby stains are new, luminescent metal chelate protein stains from Molecular Probes. The dyes are maximally excited at 470 nm and the emission peak is about 610 nm - ideal spectroscopic properties for use with the Dark Reader.
The dye is available in 3 forms:
- SYPRO Ruby Protein Gel stain allows sensitive fluorescence detection of proteins on SDS-polyacryamide, and two-dimensional gels.
- SYPRO Ruby IEF gel stain detects proteins in carrier ampholyte isoelectric focusing, immobilized pH and nondenaturing gels.
- SYPRO Ruby Protein Blot stain is appropriate for high sensitivity detection of proteins on PVDF and nitrocellulose membranes.
About 2 ng of SYPRO Ruby-stained protein can be detected directly by eye in an SDS gel using the Dark Reader and about 8 ng on a blot.
|Photographs of an SDS gel (above) and a PVDF membrane (below), kindly provided by Kiera Berggren (Molecular Probes, Inc.)
Both contain 12 lanes of 2-fold serial dilutions of Bio-Rad mid-range molecular weight markers, with the highest concentration corresponding to 2000 ng per protein band.
Both were stained with SYPRO Ruby and viewed using a Dark Reader transilluminator.
|Link to SYPRO Ruby at Molecular Probes|
Clare Chemical Research