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| A simple and sensitive fluorescence technique for the rapid determination of protein phosphorylation states in gels! | ||
| Pro-Q Diamond stain from Molecular Probes is an exciting new dye for the sensitive detection of phosphoproteins in 1-D and 2-D gels.The dye has numerous applications in kinase studies and phospho-proteomics. | ||
| A dried SDS protein gel stained with Pro-Q Diamond (kindly provided by Dr. Wayne Patton of Molecular Probes, Inc.) was photographed on a DR45 transilluminator using an Olympus 3000 digital camera and a 5 sec exposure time. The samples were alpha-casein (left side) and beta-casein. The amount of protein loaded onto the gel ranged from 500 ng down to 32 ng. Considering that Pro-Q Diamond is maximally excited around 550 nm, the detection sensitivity with the Dark Reader is remarkably good. For the most sensitive detection of faint spots in 2-D gels though, an appropriately configured laser-scanner should probably be used. |
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| Link to Pro-Q Diamond information at Molecular Probes. | ||
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Clare Chemical Research
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