Pro-Q Diamond
A simple and sensitive fluorescence technique for the rapid determination of protein phosphorylation states in gels!
Pro-Q Diamond stain from Molecular Probes is an exciting new dye for the sensitive detection of phosphoproteins in 1-D and 2-D gels.The dye has numerous applications in kinase studies and phospho-proteomics.
A dried SDS protein gel stained with Pro-Q Diamond (kindly provided by Dr. Wayne Patton of Molecular Probes, Inc.) was photographed on a Dark Reader transilluminator using an Olympus 3000 digital camera and a 5 sec exposure time.
 
The samples were alpha-casein (left side) and beta-casein. The amount of protein loaded onto the gel ranged from 500 ng down to 32 ng.
 
Considering that Pro-Q Diamond is maximally excited around 550 nm, the detection sensitivity with the Dark Reader is remarkably good. For the most sensitive detection of faint spots in 2-D gels though, an appropriately configured laser-scanner should probably be used.
The Dark Reader can be used to detect ProQ Diamond in 1D gels fairly well
Link to Pro-Q Diamond information at Molecular Probes.
Clare Chemical Research

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