Transgenic Fluorescent Fish
RFP / dsRed in transgenic Zebrafish
Not all transgenics have to be for serious research!

Zebrafish expressing red, green and yellow fluorescent proteins have recently been introduced to the home-aquarium market as GloFish™. And using a Dark Reader makes for stunning viewing.
The first few seconds of the movie show the transgenic zebrafish under normal room lighting. Then the lighting is dimmed and a Dark Reader Hand Lamp (HL32T) is used to view the brilliant fluorescence.
(Notes: The movie file is about 9 Mb. After the movie has downloaded, click the arrow at the bottom left to play. The Quicktime plugin from Apple is needed to view the movies and is available for download here.
Transgenic zebrafish expressing RFP are now available from aquarium stores throughout the USA as GloFish™. More information is available at  GloFish.com
Note: the 550 nm excitation maximum of RFP may seem to put this particular fluorophor outside the range of the blue light Dark Reader. Clearly, this is not the case. The reason is evident from a more detailed consideration of the RFP excitation spectrum which reveals substantial excitation between 400 - 500 nm. (See here for more discussion on which fluorophors work best with the Dark Reader.)

Clare Chemical Research

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