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No UV Output: No DNA Damage. |
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| DNA undergoes a number of reactions when exposed to UV light. Because the Dark Reader does not emit any UV light, the extent of DNA damage is drastically reduced compared to the damage produced by a UV device. | |||||
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| 100 ng of supercoiled (sc) plasmid was placed on either a Dark Reader (DR) or a 312 nm UV transilluminator (UV) for various times. The DNA was then digested with T4 endonuclease V, which excises T:T dimers, and run on an agarose gel and viewed. It is clear that as little as a 5 sec exposure to UV light is sufficient to convert almost 100% of the plasmid into the relaxed (rx) form and after 300 sec the DNA is completely fragmented. In contrast, a 300 sec exposure on the Dark Reader resulted in no detectable damage to the plasmid. |
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Clare Chemical Research
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